31:T6c5,

${md`The Pluslife Mini Dock is a portable nucleic acid amplification testing device used to test for COVID-19 and other respiratory illnesses. It uses a novel method, RNase HII-assisted Amplification (RHAM), which is based on the better-known Loop-Mediated Isothermal Amplification (LAMP) method. Early studies indicate similar accuracy to other rapid molecular testing devices[[1]](https://www.nature.com/articles/s41598-024-64406-9)[[2]](https://pmc.ncbi.nlm.nih.gov/articles/PMC10650960/) but at a much lower per-test price point. The Pluslife is not yet authorized by the FDA and is not intended for consumer use, but has developed a following in Germany (where it is approved for clinical use) among those interested in COVID safety, and is starting to reach a similar crowd in the US. [virus.sucks](https://virus.sucks/) maintains a third-party web app that connects to the Pluslife device and charts the raw data, making it easier to spot very weak positive results as well as common testing issues such as air bubbles. That data is then offered to the user to download. #### More about the Pluslife: - [virus.sucks: Unofficial Pluslife FAQ](https://virus.sucks/pluslife_en/) - [virus.sucks: About the analyzer app](https://virus.sucks/pluslife_en/#app) - [some examples of different test results](https://www.reddit.com/r/ZeroCovidCommunity/comments/1e4yqiw/pluslife_results_with_images/) - [what the SARS-CoV-2 test card looks like](https://covidsafeproviders.com/wp-content/uploads/anatomy-of-a-pluslife-test-card-795x1024.jpg) `}

32:Ta2f,charts = { const circleRadius = 20; const numberOfChannels = pluslifeTestData.testResult.numberOfChannels; if (numberOfChannels !== 7) { throw new Error("as far as I know, test cards always have 7 channels 🤔"); } // data setup const values = samples.map(s => s.value); const times = samples.map(s => s.time); const colors = d3.quantize(d3.interpolateRainbow, numberOfChannels + 2); const timeScale = d3.scaleLinear().domain(d3.extent(samples, d => d.time)); const samplesByTimeByChannel = d3.group(samples, d => d.time, d => d.channel); const samplesByChannel = d3.group(samples, d => d.channel); const container = d3.create("div") .style("display", "flex"); const layout = { card: { width: 240, height: 320 }, graph: { width: 560, height: 340, margin: { top: 30, right: 0, bottom: 50, left: 60 } } }; // card setup const card = cardSetup(container, layout.card, colors); const scaleRadius = d3.scaleLinear().domain(d3.extent(values)).range([2, circleRadius]); const channelPositions = d3.range(7).map((index) => ( getPointOnCircle(120, 200, outerChannelPlacementRadius, outerChannelAngles[index]) )); // graph setup const { graph, line } = graphSetup(container, layout.graph, samples); const dasharrayByChannel = d3.range(7).map((index) => { const lineLength = htl.svg``.getTotalLength(); return d3.interpolate(`0,${lineLength}`, `${lineLength},${lineLength}`); }); // draw card fluorescence at time t const circles = card.append("g") .selectAll("circle") .data(samplesByTimeByChannel.get(t)) .join("circle") .attr("cx", ([channel]) => channelPositions[channel - 1].x) .attr("cy", ([channel]) => channelPositions[channel - 1].y) .attr("r", ([channel, d]) => scaleRadius(d[0].value)) .attr("fill", ([channel]) => colors[channel - 1]) .attr("opacity", 0.5); // draw graph at time t const lines = graph.append("g") .selectAll("path") .data(samplesByChannel) .join("path") .attr("fill", "none") .attr("stroke", ([channel]) => colors[channel - 1]) .attr("d", ([channel, d]) => line(d)) .attr("stroke-dasharray", ([channel]) => dasharrayByChannel[channel - 1](timeScale(t))) .on("mouseover", function (d, i) { lines.attr("opacity", "0.25"); d3.select(this) .attr("stroke-width", "2") .attr("opacity", "1"); }) .on("mouseout", function (d, i) { lines.attr("stroke-width", "1").attr("opacity", "1"); }); return container.node(); }33:T80f,34:T5ba,attachments = ({ "2024-10-22 (negative, with bubble)": FileAttachment("pluslife_test_data-2024-10-22T20_01_50.516Z.json").json(), "2024-10-25 (negative)": FileAttachment("pluslife_test_data-2024-10-25T21_49_40@1.364Z.json").json(), "2024-11-02 (negative)": FileAttachment("pluslife_test_data-2024-11-02T15_47_53.146Z.json").json(), "2024-11-12 (negative)": FileAttachment("pluslife_test_data-2024-11-12T14_48_55.014Z.json").json(), "2024-11-19 (negative)": FileAttachment("pluslife_test_data-2024-11-19T18_02_21.949Z.json").json(), "2024-11-24 (negative)": FileAttachment("pluslife_test_data-2024-11-24T16_46_18.327Z.json").json(), "2024-12-06 (negative)": FileAttachment("pluslife_test_data-2024-12-06T22_08_07.903Z.json").json(), "2024-12-10 (negative, small bubble)": FileAttachment("pluslife_test_data-2024-12-10T16_02_06.476Z.json").json(), "2024-12-11 (negative)": FileAttachment("pluslife_test_data-2024-12-11T16_24_58.199Z.json").json(), "2024-12-13 (negative)": FileAttachment("pluslife_test_data-2024-12-13T22_35_22.394Z.json").json(), "2024-12-15 (negative)": FileAttachment("pluslife_test_data-2024-12-15T16_48_20.503Z.json").json(), "2024-12-17 (negative)": FileAttachment("pluslife_test_data-2024-12-17T17_48_07.559Z.json").json(), "2025-01-04 (negative?)": FileAttachment("pluslife_test_data-2025-01-05T18_41_29.751Z.json").json(), "2025-01-06": FileAttachment("pluslife_test_data-2025-01-06T17_46_07.861Z.json").json(), })35:T724,// controlChannel here is index based, needs to be incremented to work with chart // extracted from https://virus.sucks/_js/pluslife_app.js, would include the code here but it uses CryptoJS controlChannelByTest = [ { name: 'SARS-CoV-2', controlChannel: 3 }, { name: 'SARS-CoV-2/Flu A/Flu B', controlChannel: 3 }, { name: 'CT', controlChannel: 3 }, { name: 'NG/UU', controlChannel: 3 }, { name: 'CT/NG/UU', controlChannel: 3 }, { name: 'Flu A/Flu B/RSV', controlChannel: 3 }, { name: 'MPXV', controlChannel: 3 }, { name: 'HPV6/11', controlChannel: 3 }, { name: 'HPV16/18/45', controlChannel: 3 }, { name: 'RSV', controlChannel: 3 }, { name: 'GBS', controlChannel: 3 }, { name: 'TV', controlChannel: 3 }, { name: 'Strep A', controlChannel: 3 }, { name: 'MTB', controlChannel: 3 }, { name: 'TV/MG', controlChannel: 3 }, { name: 'CoV-2/Flu/RSV', controlChannel: 0 }, { name: 'SARS-CoV-2 (no IC)', controlChannel: 3 }, { name: 'Feline and Canine SARS-CoV-2', controlChannel: 3 }, { name: 'FPV/CPV', controlChannel: 3 }, { name: 'Toxoplasma gondii', controlChannel: 3 }, { name: 'Trichomonas Foetus', controlChannel: 3 }, { name: 'Giardia', controlChannel: 3 }, { name: 'FCM', controlChannel: 3 }, { name: 'CRQ', controlChannel: 3 }, { name: 'ASFV', controlChannel: 3 }, { name: 'CPV', controlChannel: 3 }, { name: 'FCV', controlChannel: 3 }, { name: 'FPV/CPV', controlChannel: 3 }, { name: 'FCoV/CCoV', controlChannel: 3 }, { name: 'FeLV/FIV', controlChannel: 3 }, { name: 'B.gibsoni/canis', controlChannel: 3 }, { name: 'Anaplasma/Ehrlichia', controlChannel: 3 }, { name: 'Leishmania', controlChannel: 3 }, { name: 'FPV/CPV+FCoV/CCoV', controlChannel: 3 }, { name: 'D.immitis/D.repens', controlChannel: 3 }, { name: 'G.lamblia/T.foetus', controlChannel: 3 } ]36:T635,function graphSetup(container, layout, samples) { const { width, height, margin } = layout; const graph = container.append("svg") .attr("width", width) .attr("height", height) .attr("viewBox", [0, 0, width, height]); const x = d3.scaleLinear() .domain(d3.extent(samples, d => d.time)) .range([margin.left, width - margin.right]); const y = d3.scaleLinear() .domain([ Math.floor(d3.min(samples, d => d.value) / 1000) * 1000, d3.max(samples, d => d.value) ]) .range([height - margin.bottom, margin.top]); const xAxis = graph.append("g") .attr("transform", `translate(0,${height - margin.bottom})`) .call(d3.axisBottom(x).ticks(width / 80).tickSizeOuter(0)) .call(g => g.append("text") .attr("x", width - margin.right) .attr("y", margin.bottom - 10) .attr("fill", "currentColor") .attr("text-anchor", "end") .text("time (minutes) →")); const yAxis = graph.append("g") .attr("transform", `translate(${margin.left},0)`) .call(d3.axisLeft(y).ticks(height / 40)) .call(g => g.select(".domain").remove()) .call(g => g.selectAll(".tick line").clone() .attr("x2", width - margin.left - margin.right) .attr("stroke-opacity", 0.1)) .call(g => g.append("text") .attr("x", -margin.left + 10) .attr("y", margin.top) .attr("fill", "currentColor") .attr("text-anchor", "start") .text("↑ fluorescence")); const line = d3.line() .curve(d3.curveCardinal) .x(d => x(d.time)) .y(d => y(d.value)); return { graph, line }; 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2f:{"title":"PlusLife visualization","cells":[{"id":0,"value":"# PlusLife visualization","pinned":false,"hidden":false,"mode":"md"},{"id":518,"value":"$31","pinned":false,"hidden":false,"mode":"html"},{"id":650,"value":"### TODO\n- card: pack circles with clusters of smaller circles as they scale\n - actually... circle gradient -> dither?\n- graph: what is the unit of fluorescence?\n- graph: data points or tooltips on line\n- ongoing: break up the code","pinned":false,"hidden":false,"mode":"md"},{"id":764,"value":"viewof file = Inputs.file({label: \"Test result data\"})","pinned":true,"hidden":false,"mode":"ojs"},{"id":618,"value":"viewof testSelect = Inputs.select(Object.keys(attachments), {label: \"Preset test results\", width: 300})","pinned":false,"hidden":false,"mode":"ojs"},{"id":3,"value":"pluslifeTestData = file ? await file.json() : attachments[testSelect]","pinned":false,"hidden":false,"mode":"ojs"},{"id":8,"value":"samples = pluslifeTestData.testData.samples.map(sample => ({ \n time: sample.samplingTime / 600,\n index: sample.currentDataIndex,\n channel: sample.startingChannel + 1,\n temperature: sample.samplingTemperature,\n value: sample.firstChannelResult,\n}))","pinned":false,"hidden":false,"mode":"ojs"},{"id":30,"value":"controlChannel = controlChannelByTest.find((test) => test.name === pluslifeTestData.testType).controlChannel + 1;","pinned":false,"hidden":false,"mode":"ojs"},{"id":377,"value":"viewof t = Scrubber([...new Set(samples.map(s => s.time))], {\n autoplay: false,\n initial: d3.max(samples, d => d.time),\n loop: false,\n delay: 100,\n format: (m) => `${m.toFixed(1)} minutes`\n})","pinned":false,"hidden":false,"mode":"ojs"},{"id":391,"value":"$32","pinned":true,"hidden":false,"mode":"ojs"},{"id":480,"value":"outerChannelPlacementRadius = 50","pinned":true,"hidden":false,"mode":"ojs"},{"id":476,"value":"outerChannelAngles = [-140, -182, -225, 90, 45, 2, -40];","pinned":true,"hidden":false,"mode":"ojs"},{"id":45,"value":"$33","pinned":true,"hidden":false,"mode":"html"},{"id":665,"value":"---\n\n## Appendix","pinned":false,"hidden":false,"mode":"md"},{"id":620,"value":"$34","pinned":false,"hidden":false,"mode":"ojs"},{"id":753,"value":"Promise.all(Object.values(attachments))","pinned":true,"hidden":false,"mode":"ojs"},{"id":732,"value":"$35","pinned":false,"hidden":false,"mode":"ojs"},{"id":686,"value":"function cardSetup(container, layout, colors) {\n const { width, height } = layout;\n \n const card = container.append(\"svg\")\n .html(PluslifeCardSVG)\n .attr(\"width\", width)\n .attr(\"height\", height)\n .attr(\"viewBox\", [0, 0, width, height]);\n \n const controlLegend = card.select(\"#control-legend rect\")\n .attr(\"fill\", colors[controlChannel - 1]);\n\n return card;\n} ","pinned":false,"hidden":false,"mode":"ojs"},{"id":677,"value":"$36","pinned":false,"hidden":false,"mode":"ojs"},{"id":471,"value":"PluslifeCardSVG = {\n var serializer = new XMLSerializer();\n return serializer.serializeToString(PluslifeCard);\n}","pinned":false,"hidden":false,"mode":"ojs"},{"id":162,"value":"// unfortunately this is 98% copied and pasted from the Google AI Overview suggestion. feels gross tbh! \nfunction getPointOnCircle(centerX, centerY, radius, angle) {\n const radians = angle * Math.PI / 180;\n const x = centerX + radius * Math.cos(radians);\n const y = centerY + radius * Math.sin(radians);\n return { x, y };\n}","pinned":true,"hidden":false,"mode":"ojs"},{"id":379,"value":"import {Scrubber} from \"@mbostock/scrubber\"","pinned":true,"hidden":false,"mode":"ojs"}],"files":"$W37","stdlib":"1","titling":"auto"}